sh sy5y neuroblastoma cells  (ATCC)

Journal: iScience

Article Title: CK1δ-dependent SNAPIN dysregulation drives lysosomal failure in HIV-1 Vpr-exposed neurons: A targetable mechanism in HAND

doi: 10.1016/j.isci.2025.114544

Figure Lengend Snippet: HIV-1 Vpr impairs lysosomal degradation, leading to cargo accumulation in neuronal lysosomes SH-SY5Y-derived neurons expressing LAMP1-mCherry were treated with recombinant HIV-1 Vpr (8 nM) and analyzed by confocal microscopy. (A) Lipid accumulation detected by BODIPY (green) colocalized with LAMP1 (red) after Vpr treatment. (B) MitoTracker-labeled mitochondrial remnants (green) persisted within lysosomes after Vpr exposure. (C) Thioflavin-T (green) revealed enhanced amyloid-like deposits in Vpr-treated lysosomes. (D) α-synuclein (green) showed increased lysosomal association following Vpr treatment. Scale bars: 20 μm. Representative qualitative images are shown; no statistical analysis was performed for this figure.

Article Snippet: SH-SY5Y neuroblastoma cells , ATCC , CRL-2266.

Techniques: Derivative Assay, Expressing, Recombinant, Confocal Microscopy, Labeling

Journal: iScience

Article Title: CK1δ-dependent SNAPIN dysregulation drives lysosomal failure in HIV-1 Vpr-exposed neurons: A targetable mechanism in HAND

doi: 10.1016/j.isci.2025.114544

Figure Lengend Snippet: HIV-1 Vpr increases neuronal CK1δ expression Immunofluorescence of CK1δ (green) in SH-SY5Y-derived neurons treated with vehicle, Vpr, LH846, or Vpr + LH846. LAMP1 (red) labels lysosomes; nuclei are DAPI-stained (blue). Representative qualitative images are shown. Scale bars: 10 µm.

Article Snippet: SH-SY5Y neuroblastoma cells , ATCC , CRL-2266.

Techniques: Expressing, Immunofluorescence, Derivative Assay, Staining

Journal: iScience

Article Title: CK1δ-dependent SNAPIN dysregulation drives lysosomal failure in HIV-1 Vpr-exposed neurons: A targetable mechanism in HAND

doi: 10.1016/j.isci.2025.114544

Figure Lengend Snippet: CK1δ inhibition restores lysosomal distribution disrupted by HIV-1 Vpr SH-SY5Y neurons expressing LAMP1-mCherry and SNAPIN wild-type (WT) or S50D were treated with Vpr ± LH846 (1.5 μM). Radial intensity profiles quantified lysosomal distance from nuclei. Scale bars: 10 μm.

Article Snippet: SH-SY5Y neuroblastoma cells , ATCC , CRL-2266.

Techniques: Inhibition, Expressing

Journal: Alzheimer's & Dementia

Article Title: NRN1 as a therapeutic target for Alzheimer's disease

doi: 10.1002/alz.71149

Figure Lengend Snippet: Abcam ab64186 recognizes NRN1 protein in mouse neuroblastoma cells. (A) Representative Western blot of human embryonic kidney (HEK) 293T, Neuro‐2a (N2a) mouse neuroblastoma, and SH‐SY5Y human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;

Article Snippet: SH‐SY5Y human neuroblastoma cell lines (Catalog No.: CRL‐2266, ATCC) were maintained in a 1:1 mixture of DMEM/F12 (Catalog No.: 11330057, Thermo Fisher Scientific) and Eagle's Minimum Essential Medium (Catalog No.: 50‐238‐2632, Fisher Scientific) with 10% FBS and 1% penicillin‐streptomycin.

Techniques: Western Blot, Transfection, Control, Small Interfering RNA

Journal: Science Advances

Article Title: Optoacoustically augmented magnetic guidewire for radiation-free minimally invasive therapies

doi: 10.1126/sciadv.aea0201

Figure Lengend Snippet: ( A ) Experimental setup for remote RF hyperthermia to kill the cancer cells. ( B ) Infrared temperature images of OptoMaG. ( C ) The temperature elevation in the magnetic tip of OptoMaG was induced by RF heating at distances of 1 and 5 cm. ( D ) Comparison of the conditions of three experimental groups of SH-SY5Y cells after 72 hours of culturing with RF heating. Scale bars, 100 μm. ( E ) The condition of three experimental groups of SH-SY5Y cells after various durations of RF heating. ( F ) SH-SY5Y cell survival in three groups after RF induction heating. ( G ) Schematic of PDT for the treatment of glioblastoma using OptoMaG, providing a blue light source. 5-ALA–PDT induces cell death via apoptosis/necrosis. 5-ALA accumulates in the mitochondria and forms protoporphyrin IX (PPIX) using the heme synthesis pathway. Activation with light of a specific wavelength causes a photodynamic reaction, producing ROS, which in turn leads to cell death. Designed in Adobe Illustrator. ( H ) 5-ALA–induced PPIX generation in the presence of 5-ALA or OptoMaG’s blue light. PPIX distribution in the cells can be visualized by its red fluorescence. ( I ) ROS generation after PDT on the SH-5SY distribution in the cells can be visualized by its red fluorescence. ( J ) Visualization of intracellular ROS 0, 2, and 6 hours (h) after OptoMaG’s blue light irradiation in SH-SY5Y. ( K ) SH-SY5Y cell survival after ALA-PDT in the presence of ALA or OptoMaG’s blue light.

Article Snippet: SH-SY5Y cells were purchased from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (American Type Culture Collection, CRL-2266, RRID: CVCL_0019).

Techniques: Comparison, Activation Assay, Fluorescence, Irradiation